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. 2022 May 25;12:8857. doi: 10.1038/s41598-022-12785-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology of oocyte–cumulus–granulosa complexes (OCGCs) before and after 12 days of in vitro growth (IVG) culture. (a) Isolated OCGC before IVG culture. (b) Surviving OCGC without antrum formation in the granulosa cell layer after 12 days of IVG culture. (c) Surviving OCGC with antrum formation (white arrowhead) in the granulosa cell layer. (d) Degenerated OCGCs after 12 days of IVG culture. Scale bar = 100 µm.