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. 2022 May 25;12:8857. doi: 10.1038/s41598-022-12785-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of cysteine supplementation on the GSH levels of oocytes exposed to high temperature during in vitro growth (IVG) culture. (a) Representative fluorescent photomicrographs of IVG oocytes detected with CellTracker Blue. The intracellular GSH levels were evaluated on day 12 of IVG culture in the cysteine untreated group (left panels) and cysteine treated group (right panels). Scale bar = 50 µm. (b) The relative fluorescent intensity for GSH levels from the cysteine untreated and treated group after day 12 of IVG culture. The fluorescent intensity of GSH was measured using a total of 37 oocytes (three replicates each). Numbers in the bar graph indicate the number of oocytes, while the number of replicates is shown in parentheses. The fluorescence intensity of oocytes in the cysteine treated group was normalized to those in the cysteine untreated group. Error bars indicate SEM. *An asterisk indicates a significant difference between the cysteine untreated and treated groups (P < 0.05).