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. 2022 May 25;12:8835. doi: 10.1038/s41598-022-12749-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of novel differentially expressed markers by immunohistochemistry of young adult mouse heart. IHC of 10-week-old male mouse heart, double labelling with novel differentially expressed markers identified by proteomic analysis of cell cultures (Figs. 6 and Supplemental Fig. S3). Novel markers (Alexa Fluor 594) are double-labelled with classical cell-specific markers (Alexa Fluor 488): CD31 for ECs, NG2 for PCs, calponin-1 for VSMCs; scale bar (DAPI, Alexa Fluor 488 and 594, merged), 20 µm, scale bar (inset), 5 µm.