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. 2022 May 25;13(5):497. doi: 10.1038/s41419-022-04960-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A Protein expression of FAT10 in noncancerous pancreatic ductal epithelial cells and PC cell lines was analyzed by western blotting. B The mRNA expression of FAT10 in normal pancreatic ductal epithelial cells and PC cell lines was analyzed by qRT–PCR. C Viabilities of PC cells were determined in response to different concentrations of GEM. Inhibition curves were fitted by nonlinear regression, and GEM IC50s were calculated using GraphPad Prism 8 software. D Western blotting was used to analyze expression levels of FAT10 in GEM-resistant (GR) PC cells. Data represent the mean ± SD of triplicate experiments and were statistically analyzed with Student’s t-test, *p < 0.05, **p < 0.01.