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. 2022 May 25;13(5):497. doi: 10.1038/s41419-022-04960-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Spearman correlation analysis of the correlation between FAT10 (UBD) and EMT pathway score. FAT10 expression is represented by the abscissa, and the EMT pathway score is represented by the ordinate. A density curve to the right represents the trend in the distribution of pathway scores, a density curve to the upper part represents the trend in the distribution of gene expression. The top part shows the p-value, correlation coefficient, and correlation calculation method. B Immunofluorescence analysis of the effect of inhibiting FAT10 on the expression of EMT-related proteins (E-cadherin and Vimentin) in PC cells. Scale bar, 50 μm. C–F GEM-resistant PC cell lines (PANC-1/GR and BxPC-3/GR) were transfected with interfering plasmid sh-FAT10#2 and or EMT activator (TGF-β, 10 ng/mL) for 48 h. C, E Western blot analysis was used to observe the expression of EMT-related proteins in each treatment group. D, F Cell viability experiments were used to calculate the half-inhibitory concentration (IC50) of GEM-treated PC-resistant cells in each treatment group. Data represent the mean ± SD of triplicate experiments and were statistically analyzed by one-way ANOVA. *p < 0.05, **p < 0.01, ***p < 0.001.