Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 25;13(5):497. doi: 10.1038/s41419-022-04960-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — A Co-IP was used to detect the interaction between FOXM1 and FAT10 in PANC-1 and BxPC-3 cells. HC, heavy chain. B MG132 (10 μM) was added to PANC-1 and BxPC-3 cells, shFAT10 or HA-FAT10 plasmid was transfected at the same time, and then co-IP was used to detect the levels of ubiquitin bound to FOXM1 protein. C PANC-1 and BxPC-3 cells were transfected with different amounts of HA-FAT10 plasmid, and the level of FAT10 binding to FOXM1 protein was detected by co-IP. D PANC-1 and BxPC-3 cells were transfected with different amounts of HA-FAT10 plasmid, and the level of Ub binding to FOXM1 protein was detected by co-IP. E Docking conformation of the first ranking score. Three-dimensional structure of FAT10 and FOXM1. FAT10 is shown in green. FOXM1 is shown in cyan. F The Flag-FOXM1 plasmid was transfected into PANC-1 and BxPC-3 cells, the HA-FAT10 or HA-FAT10ΔGG plasmid was transfected at the same time, and then the protein level of Flag-FOXM1 bound to the HA-tagged protein was detected by co-IP. HC, heavy chain.