TABLE 1.
Substrate or product | Substrate concn (mol/100 mol) | Carbon concn (mol) | Available hydrogen concn
|
|
---|---|---|---|---|
mol of H/mol | mol of H/100 mol of lactose | |||
Substrates | ||||
Lactose | 100 | 1,200 | 48 | 4,800 |
Lactic acid | 3 | 9 | 11 | 33 |
CO2 | 346 | 346 | 0 | 0 |
Products | ||||
Succinic acid | 243 | 972 | 14 | 3,402 |
Acetic acid | 111 | 222 | 8 | 888 |
Formic acid | 47 | 47 | 2 | 94 |
Carbon and electron balance were determined as described by Zeikus et al. (34), except the carbon and electron balance in cells was not included. CO2 was supplied as magnesium carbonate (35 g/liter). Note that 80% of the carbon (moles) and 92% of the available hydrogen (moles of hydrogen per 100 moles of lactose) were recovered.