Table 1.
Details of bacterial strains used in this study.
| Sl. no. | Strain/isolate | Genotype | Reference |
|---|---|---|---|
| 1 | Bacillus megaterium G18 | Wild type | 1 |
| 2 | E. coli TOP10 | F-, mcrA, Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 Δ lacX74 recA1 araD139 Δ(araleu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen™, USA |
| 3 | E. coli S17.1 | pro, res− hsdR17 (rK− mK+) recA− with an integrated RP4-2-Tc::Mu-Km::Tn5, Tpr | 2 |
| 4 | E. coli K12- JW0233-2 (CGSC#: 8468) | F-, Δ(araD-araB)567, ΔproA761::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514 | CGSC, USAa |
| 5 | E. coli K12-JW0232-1 (CGSC#: 8467) | F-, Δ(araD-araB)567, ΔproB760::kan, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514 | CGSC, USA |
| 6 | E. coli K12-JW0377-1 (CGSC#: 8554) | F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), ΔproC751::kan, λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514 | CGSC, USA |
| 7 | Wild type, E. coli K12-MG1655, CGSC#: 6300 | F-, λ-, rph-1 | CGSC, USA |
| 8 | Bacillus megaterium BA5 | Neor | This study |
| 9 | Bacillus megaterium 13A (ΔproC) | ErmR | This study |
| 10 | Bacillus megaterium ΔproC (pHT01-proC+) | Chloramphenicol resistance (CmR), ErmR | This study |
aCGSC: Coli Genetic Stock Center, Yale University, USA.