Table 2.
Details of plasmids used in this study.
| Sl. no. | Vectors | Characteristics | Reference |
|---|---|---|---|
| 1. | pSUP:Tn5:mob | pBR325::Tn5-mob with ampicillin [Apr], chloramphenicol [Cmr], and kanamycin resistance [Kmr] and a P-type-specific recognition site for mobilization | 2 |
| 2. | pMUTIN4 | Integration vector for Bacillus spp. containing Apr and ErmR (erythromycin resistance) | BGSC, USAa |
| 3. | pMutProCi | Derivative of pMUTIN4 containing PCR amplified fragment proCi, Apr and ErmR | This study |
| 4. | pHT01 | Expression vector for Bacillus spp. with Apr and Cmr | MoBiTec, USA |
| 5. | pHT01-proC | Derivative of pMUTIN4 containing PCR amplified full length proC gene, Apr and Cmr | This study |
aBGSC: Bacillus Genetic Stock Center, Ohio State University, USA.