Table 2.
RT-PCR and replication assay results for viruses in honey bees and V. destructor. Pooled V. destructor and honey bee samples were screened for eight viruses (DWV-A, DWV-B (VDV-1), BQCV, KBV, IAPV, VDV-2, VDV-3 and VDV-5). Positive test results are indicated with ( +) while (−) indicates that no band was observed. A positive result for the negative-strand detection assay is indicative that the virus is parasitising the host cells. Primer pairs used in this analysis are shown in Supplementary Table S4.
| Virus or virus strain | RT-PCR detection | Negative-strand detection | ||
|---|---|---|---|---|
| Honey bee | V. destructor | Honey bee | V. destructor | |
| DWV-A | + | + | + | + |
| DWV-B (VDV-1) | − | − | − | − |
| BQCV | + | + | − | − |
| KBV | + | + | + | + |
| IAPV | − | − | − | − |
| VDV-2 | − | + | − | + |
| VDV-3 | − | − | − | − |
| VDV-5 | − | + | − | + |