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. 2022 May 25;12:8813. doi: 10.1038/s41598-022-12679-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferation of primary rat hepatocytes after 24-h treatment of retrorsine in cell fibers. (a, b) Representative dark-field images of primary rat hepatocytes cultured in cell fibers (n = 5 for each group) for 2 days, 3 days, 4 days, and 7 days using 3T3CM. Cell fibers encapsulating primary hepatocytes were divided into two groups: (a) in one group the hepatocytes were not treated with retrorsine, (b) while in the other group they were treated with retrorsine for 24-h after 2 days of culture. Scale bars; 100 µm. (c) Time course of total cell number of primary rat hepatocytes cultured in cell fibers using 3T3CM either (c) with or (d) without 24-h retrorsine treatment after 2 days of culture (n = 5 per data point). Error bars denote mean ± SD.