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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: J Nutr Biochem. 2022 Mar 26;105:108998. doi: 10.1016/j.jnutbio.2022.108998

Fig. 1. Piceatannol inhibits lipolysis by lowering ATGL, CGI-58 and PLIN1 levels in adipocytes.

Fig. 1.

Differentiated 3T3-L1 adipocytes (A-D, G, H) and murine brown adipocytes (E.F) were incubated with piceatannol (0–50 μM) or resveratrol (0–50 μM) in the presence or absence of isoproterenol (10 μM) in DMEM containing 1% fatty acid-free bovine serum albumin for 90 min or at the indicated time points. The medium was collected to measure the released glycerol (A, E) and FFA (B, G) levels as described in the Experimental Section. Data are presented as means ± SEM. (n=3, *, p < 0.05; **, p < 0.01). Total cellular proteins at 90 min or the indicated time points were extracted from the differentiated 3T3-L1 adipocytes (C, D, H) and brown adipocytes (F), and analyzed by Western blot analysis for ATGL, CGI-58, PLIN1, p-HSL, HSL and β-actin. iso: isoproterenol, Pic: piceatannol, Res: resveratrol, Cont: control, Und: undifferentiated.