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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: J Nutr Biochem. 2022 Mar 26;105:108998. doi: 10.1016/j.jnutbio.2022.108998

Fig. 2. Piceatannol promotes autophagy-lysosome pathway-dependent degradation of a lipolytic protein cluster of ATGL, CGI-58, and PLIN1 in adipocytes.

Fig. 2.

Differentiated 3T3-L1 adipocytes were incubated with piceatannol (0–50 μM) in the presence or absence of various concentration of MG132 (A, B), 10 mM 3-MA (C, D) and 10 μM CQ (E, F) in DMEM containing 1% fatty acid-free bovine serum albumin for 90 min. The medium was collected to measure the released glycerol (A, C, E) levels as described in the Experimental Section. Data are presented as means ± SEM. (n=3, *, p < 0.05). Total cellular proteins isolated from these cells were analyzed by Western blot analysis for ATGL, CGI-58, PLIN1 and β-actin (B, D, F).