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. Author manuscript; available in PMC: 2023 May 23.
Published in final edited form as: Curr Biol. 2022 Apr 6;32(10):2272–2280.e6. doi: 10.1016/j.cub.2022.03.038

Figure 3. pparγ transcripts and Pparγ protein is upregulated in cavefish livers.

Figure 3.

A) pparγ mRNA expression level comparison between surface fish and Pachón cavefish under two feeding conditions: 4-day fasted and refed. TPM indicates transcript per million reads (n = 3 for each group, *** p < 0.001). B) Immunostaining of Pparγ (magenta), E-cadherin (yellow), and DAPI (turquoise) in liver sections of surface fish and Pachón cavefish. No primary ab indicates no primary antibody control. Scale bar = 30 μm. C) Quantification of mean fluorescent intensity of Pparγ staining (n=3 for surface fish and Pachón livers. 187–317 hepatocytes were randomly selected from each fish liver sample for intensity measurement (Wilcoxon test, * p < 0.05). D) Venn diagram of Pparγ ChIP-seq peaks within 3kb of predicted transcription start sites in surface and Pachón cavefish livers. E) Comparison of Pparγ ChIP-seq peak height (in log2 normalized read number) between surface fish and Pachón cavefish (576 peaks with Pparγ canonical binding sites. wilcoxon test, ** p < 0.01). F) Examples of Ppary ChIP-seq peaks on known lipogenesis target genes (mgat1a, cd36).