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. Author manuscript; available in PMC: 2023 May 23.
Published in final edited form as: Dev Cell. 2022 Apr 27;57(10):1226–1240.e8. doi: 10.1016/j.devcel.2022.04.003

Figure 1: Single-cell RNA-sequencing (scRNA-seq) reveal a developmental hierarchy within RMS.

Figure 1:

A-B, During fetal myogenesis, mesodermal cells of the somite migrate to form skeletal muscle throughout the body (A). During that migration, these cells undergo stepwise differentiation typified by the transient expression of myogenic regulatory factors (B). C-D, Photomicrographs of an embryonal RMS tumor, SJRHB030680_R1 (C) and an alveolar RMS tumor, SHRHB031320_D1. Left, H&E staining. Right, Myogenin (MYOG) immunohistochemistry (IHC) with 20X magnification, inset, 80X magnification. E-F, UMAP visualization of 3,973 malignant cells from SJRHB030680_R1 (E) and 2,414 malignant cells from SJRHB031320_D1 (F). Cells are colored based on expression of MEOX2 (left), MYF5 (center), and MYOG (right). G-H, RNA velocity analysis of SJRHB030680_R1 (G) and SJRHB031320_D1 (H). Abbreviations: ERMS, embryonal rhabdomyosarcoma; UMAP, uniform manifold approximation and projection. Scale bars: C,D, 100 μm.