Figure 1. CRLF2 overexpression enhances B-lymphoid colony formation and reduces B cell maturation, with a greater fold change in the Dp16 background.

(A) CRLF2 overexpression in BM HSPCs from both WT and Dp16 mice increased B-lymphoid colony growth compared to GFP control. Histogram depicts colony counts from six samples (two technical replicates across three independent experiments, Student’s t-test, *p<0.01, **p<0.001). Error bars indicate standard deviation. (B) CRLF2 overexpression in WT and Dp16 HSPCs grown in B cell-differentiation conditions resulted in an increased percentage of the less-differentiated pre-pro-B cells. This effect was greater in the Dp16 versus WT genetic background. Histogram shows percentage of pre-pro-B cells in the GFP⁺ gate of each experimental group, with mean values from three independent experiments with two biological replicates each (Student’s t-test, *p<0.05, **p<0.001). Error bars indicate standard deviation.