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Immunofluorescence of ERα in pre-implantation embryos. Scattered ERα expression was detected in the cytoplasm as well as the nucleus (co-stained with DAPI) of 1-cell stage embryos to blastocysts. ERα: green; DAPI: blue. Scale bar = 50 μm (A) Unstained; (B) stained with DAPI; (C) stained with primary and secondary antibodies for ERα; (D) stained with only secondary antibodies for ER α. Representative images from three experiments using biologically different samples (n ≥ 15) are shown.
