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. 2022 May 12;12:808888. doi: 10.3389/fonc.2022.808888

Figure 4.

Figure 4

circSAFB2 serves as a sponge for miR-620. (A) Localization of circSAFBin macrophages after treatment with exosomes detected by FISH; (B) Schematic representation of the 3′-UTR of circSAFB2 with the predicted target site for miR-620. The mutant site of circSAFB2 3′-UTR is indicated (without line); (C) A luciferase reporter analysis was performed to examine the binding ability between circSAFB2 and miR-620. Reporter constructs containing circSAFB2wt or circSAFB2mut at the predicted miR-620 target sequences were co-transfected into macrophages, along with miR-620 mimics or miR-NC; (D) Lysates prepared from macrophages overexpressing circSAFB2 were incubated with biotinylated probes against circSAFB2 before performing an RNA pull-down assay. qRT-PCR was used to determine the level of circSAFB2 and miR-620; (E) qRT-PCR analyses of miR-620 expression in macrophages after exosome treatment (Exo-769-P and Exo-ACHN); (F) qRT-PCR analysis of miR-620 expression in macrophages treated with exosomes and exosomes knocked down circSAFB2. All data are reported as mean ± SD; n=3, ***P < 0.001. ns, no significance.