Figure 6.

RCC-derived exosomal circSAFB2 facilitates the progression of RCC in vitro and in vivo by inducing macrophage M2 polarization. (A) The proliferation capacity of ACHN cells co-cultured with macrophages subjected to the indicated treatment determined by the CCK8 assay at 0, 24, 48, and 72 hours; (B, C) Migration and invasion capacity of Huh7 cells co-cultured with macrophages subjected to the indicated treatment detected by Transwell; (D, E) Bioluminescence imaging of mice after ACHN cell injection into the tail vein alone or ACHN cells co-injected with macrophages subjected to the indicated treatment (n=6 per group). Metastasis was monitored and imaged by bioluminescence using an in-vivo imaging system. All data are indicated as the mean ± SD; n=3, **P < 0.01, ***P < 0.001. ns, no significance.