Figure 6.

CAR Tregs persist and home to bone marrow following adoptive transfer into antigen-negative host

(A) Following adoptive transfer of Bw6-specific CAR Tregs into a non-preconditioned, antigen-negative recipient, peripheral blood was stained with Bw6 tetramer to detect Bw6-specific CAR Tregs. Dot plots show CD4⁺ CD8⁻ FoxP3⁺ gated cells in peripheral blood at the indicated time points.

(B and C) Detection of Bw6-specific CAR molecule on CD4⁺ CD8⁻ FoxP3⁺ gated cells in peripheral blood (B) or bone marrow (C) at the indicated time points following adoptive transfer of Bw6-specific CAR Tregs into rapamycin and IL-2 preconditioned, antigen-negative animal.

(D) Flow cytometry of CXCR4 expression from three separate Bw6-specific CAR Treg expansion products and unstimulated CD4s.