REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD4-BV421	Biolegend	Cat#317434; Clone: OKT4; RRID:AB_2562134
CD4-AF488	Biolegend	Cat#317420; Clone: OKT4; RRID:AB_571939
CD4-PerCP-Cy5.5	Biolegend	Cat#317428; Clone: OKT4; RRID:AB_1186122
CD4-BV605	Biolegend	Cat#317438; Clone: OKT4; RRID:AB_11218995
CD8-BV510	Biolegend	Cat#301048; Clone: RPA-T8; RRID:AB_2561942
CD25-BV421	Biolegend	Cat#302630; Clone: BC96; RRID:AB_11126749
CD127-PE	Biolegend	Cat#351301; Clone: A019D5; RRID:AB_10720815
CXCR4-APC	Biolegend	Cat#306510; Clone: 12G5; RRID:AB_314616
CD45RA-FITC	BD Biosciences	Cat#556626; Clone: 5H9; RRID:AB_396498
CD3-PerCP-Cy5.5	BD Biosciences	Cat#552852; Clone: SP34-2; RRID:AB_394493
Goat α-Mouse IgG F(ab)2-AF488	Jackson Immunoresearch	Cat#114-454-072
Fixable Viability Dye eFluor 780	eBioscience	Cat#65-0865-18
CTLA-4-BV786	BD Biosciences	Cat#563931; Clone: BNI3; RRID:AB_2738491
Helios-PE/Cy7	Biolegend	Cat#137236; Clone: 22F6; RRID:AB_2565990
FoxP3-PE/Dazzle 594	Biolegend	Cat#320126; Clone: 206D; RRID:AB_2564025
HLA-B∗07:02 HIV nef TPGPGVRYPL-PE	MBL International	Cat#TS-M054-1
HLA-A∗02:01 HIV gag SLYNTVATL-APC	MBL International	Cat#TB-M027-2
IL2-APC	BD Biosciences	Cat#554567; Clone: MQ1-17H2; RRID:AB_398571
TNFα-PE/Cy7	BD Biosciences	Cat#557647; Clone: MAB11; RRID:AB_396764
IFNγ-FITC	BD Biosciences	Cat#552882; Clone: 4S.B3; RRID:AB_394511
MIP1β-PerCP-Cy5.5	BD Biosciences	Cat#560688; Clone: D21-1351; RRID:AB_1727567
IL-10-PE/Dazzle594	Biolegend	Cat#501426; Clone: JES3-9D7; RRID:AB_2566744
LAP-APC	Biolegend	Cat#349706; Clone: TW5-6H10; RRID:AB_10680787
CD25-PE/Dazzle 594	Biolegend	Cat#302646; Clone: BC96; RRID:AB_2734260
CD8-PE	Biolegend	Cat#301008; Clone: RPA-T8; RRID:AB_314126
CD86-BV421	Biolegend	Cat#374212; Clone: BU63; RRID:AB_2728394
Streptavidin-PE	BD Biosciences	Cat#554061; RRID:AB_10053328
Cynomolgus CD3 epsilon protein	ACRO Biosystems	Cat#CDE-C5254
Biotinylated Human CD3 epsilon protein	ACRO Biosystems	Cat#CDE-H82E1
His Tag-AF647	Biolegend	Cat#652513; Clone: J099B12; RRID:AB_2716153
Active Caspase-3-BV650	BD Biosciences	Cat#564096; Clone: C92-605; RRID:AB_2738589
Biological samples
Fetal Bovine Serum	Avantor Seradigm	Cat#97068-085
Human Serum Albumin	CSL Behring	N/A
Chemicals, peptides, and recombinant proteins
Penicillin-Streptomycin	Gibco	Cat#15140122
Proleukin (aldesleukin)	Clinigen	N/A
Percoll	Millipore Sigma	Cat#P4937
ACK Lysing Buffer	Quality Biological	Cat#118-156-101
Phorbol 12-myristate 13-acetate (PMA)	Sigma Aldrich	Cat#P1839
Ionomycin	Sigma Aldrich	Cat#407950
GlutaMAX Supplement	Gibco	Cat#35050061
HEPES	Gibco	Cat#15630080
RPMI 1640	Gibco	Cat#11875085
Normocin	Invivogen	Cat#ant-nr-1
OpTmizer T Cell Expansion Medium	Gibco	Cat#A1048501
Rapamycin (Rapamune)	Pfizer	N/A
Rapamycin	LC Laboratories	R-5000
Streptozotocin (Zanosar)	Pharmacia & Upjohn	N/A
Critical commercial assays
FlowPRA single antigen HLA Class I Beads	One Lambda	Cat#FL1HD02, Group 2
FlowPRA single antigen HLA Class I Beads	One Lambda	Cat#FL1HD08, Group 8
True-Nuclear Transcription Factor Buffer Set	Biolegend	Cat#424401
T Cell Activation/Expansion Kit, non-human primate	Miltenyi Biotec	Cat#130-092-919
CellTrace Violet	Molecular Probes	Cat#C34557
CellTrace CFSE	Molecular Probes	Cat#C34554
CellTrace Far Red	Molecular Probes	Cat#C34564
CountBright Absolute Counting Beads	Invitrogen	Cat#C36950
GolgiPlug Protein Transport Inhibitor	BD Biosciences	Cat#555029
DNeasy Blood & Tissue Kit	Qiagen	Cat#69504
Monkey FOXP3 Assay	Epigendx	Cat#ADS783-FS4
Fixation Medium A	Invitrogen	Cat#GAS001S100
Fixation Medium B	Invitrogen	Cat#GAS001S100
Lipofectamine 2000	Invitrogen	Cat#11668027
C-peptide specific ELISA	ARUP Laboratories	N/A
Insulin (Humulin R)	Eli Lilly and Company	N/A
Insulin (Glargine)	Eli Lilly and Company	N/A
CMRL 1066	Corning	Cat#98-304-CV
Valgancyclovir (Valcyte)	Genentech	N/A
Experimental models: Cell lines
K-562	ATCC	CCL-243