Figure 3.

Transitory and terminally differentiated PD-1⁺ CD8⁺ T cells infiltrate human brain metastases

(A) UMAPs of all 22,828 PD-1⁺ CD8⁺ T cells sorted from 5 brain metastases and naive cells sorted from patient-matched blood samples. Hierarchical clustering is shown at right.

(B) Distribution of each patient’s PD-1⁺ CD8⁺ T cells among metaclusters.

(C) Distribution of PD-1⁺ CD8⁺ T cells among metaclusters for each primary tumor type.

(D) Phenotype of PD-1⁺ CD8⁺ T cells from brain metastasis and matched circulating naive cells for each patient.

(E) Expression of selected genes in each metacluster. Numbers above each violin indicate the percentage of cells in each metacluster expressing the gene.

(F) Expression of selected genes projected on UMAP.

(G) Relative expression of selected genes in each metacluster.

See also Figures S3 and S4.