Figure 6.

Genes associated with the terminally differentiated CD8⁺ T cell phenotype are preferentially expressed within the tumor parenchyma of a melanoma brain metastasis

(A) Hematoxylin-and-eosin-stained section of a melanoma brain metastasis (patient 16).

(B) Spatial location of capture spots, colored by transcriptional cluster.

(C) UMAP and clustering of capture areas by transcriptional phenotype.

(D) Expression of selected genes within the tissue section. White indicates no detection of the indicated gene in a given capture area. A spatial legend is given at bottom right.

(E) Normalized expression density of selected genes in each tissue cluster.

(F) Gene-expression differences between tumor (clusters 3, 4, and 5) and peritumoral inflammation (clusters 1 and 7).

(G) Differential gene expression between inflammation-adjacent tumor (cluster 5) and the remainder of tumor (clusters 3 and 4).

See also Figures S8–S14.