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. 2022 May 12;12:764621. doi: 10.3389/fonc.2022.764621

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Copyright © 2022 Song, Li, Chen, Zhang, Yang, Zhang, Song, Miao, Chang and Shi

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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FAM222A-AS1 knockdown inhibited CRC cell proliferation, migration and invasion in vitro. (A) The expression of FAM222A-AS1 in normal colorectal cell (FHC) and six CRC cells (HT29, Caco2, SW620, SW480 and HCT116). (B) The expression of FAM222A-AS1 in SW480 and HCT116 after transfection with si-NC or si-FAM222A-AS1 were detected by RT-PCR. (C-D). The effects of FAM222A-AS1 knockdown on the proliferation of SW480 and HCT116 cells were examined by CCK-8 assay (C) and colony formation assays (D). Experiments were performed in triplicate. (E) Transwell assays were used to detect the migration and invasion of SW480 and HCT116 cells after FAM222A-AS1 knockdown. Columns are the average of three independent viewing fields. (F) RT-PCR was used to determine the knockdown efficiency of the FAM222A-AS1-shRNA vector in HCT116 and SW620 cells after induced by Dox (2.5 ug/mL). (G) The effects of FAM222A-AS1 knockdown (induced by Dox) on the proliferation of SW620 and HCT116 cells were examined by CCK-8 assay. Experiments were performed in triplicate. (H) The effects of FAM222A-AS1 knockdown (induced by Dox) on the proliferation of HCT116 cells were examined by colony formation assays. (I) Transwell assays were used to detect the migration and invasion of HCT116 cells after FAM222A-AS1 knockdown induced by Dox. Columns are the average of three independent viewing fields. (J) RT-PCR was used to determine the overexpression efficiency of the FAM222A-AS1-overexpression vector in Caco2 and HT29 cells after induced by Dox (2.5 ug/mL). (K) The effects of FAM222A-AS1 overexpression (induced by Dox) on the proliferation of Caco2 and HT29 cells were examined by colony formation assay. (L) Scratch assays were used to detect the migration of Caco2 and HT29 cells after FAM222A-AS1 overexpression induced by Dox. Columns are the average of three independent viewing fields.NC, si-NC; siRNA, si-FAM222A-AS1. Caco2-OE, Caco2 stable cells transinfected with FAM222A-AS1 overexpression vectors. HT29-OE, HT29 stable cells transinfected with FAM222A-AS1 overexpression vectors; -Dox, the control group without Dox; +Dox, FAM222A-AS1 was knockdown or overexpressed induced by Dox (2.5μg/mL). (Ns, no significantly difference; *P<0.05, **P < 0.01 and ***P < 0.001).