FIGURE 4.

Activation of ferroptosis impaired DHQ-induced anti-fibrosis effects in vitro (A) The protein expressions of GPX4 in activated HBE cells were measured by western blotting. (B) The analysis of cell viability and major biomarkers of ferroptosis (iron, GSH, ROS, MDA and 4-HNE) in activated HBE cells. (C) The protein expressions of α-SMA, collagen I and fibronectin in MRC-5 cells stimulated with conditioned medium of HBE cells were measured by western blotting. (D) The analysis of real time-PCR of α-SMA, collagen I and fibronectin in MRC-5 cells. Data are shown as mean ± SD (n = 3). #p < 0.05, ##p < 0.01 vs. the SiO₂ group; *p < 0.05, **p < 0.01 vs. the SiO₂+DHQ group.