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. 2022 May 12;13:839390. doi: 10.3389/fimmu.2022.839390

Figure 3.

Figure 3

Effect of ethanol on CD4+ T cell glucose metabolism. (A) Representative FACS plots of GLUT1 expression in DIFF and DIFF + EtOH CD4+ T cells. (B) Ethanol increased CD4+ T cell GLUT1 expression. (C) Representative FACS histograms of 2-NBDG uptake in DIFF and DIFF + EtOH CD4+ T cells. (D) Ethanol increased glucose uptake within differentiated CD4+ T cells. (E) All experimental groups were analyzed using the Glycolytic Stress test. Ethanol increased glycolysis (F) and glycolytic capacity (G) within differentiated groups. MFI = mean fluorescent intensity, 2-NBDG = 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose, ECAR = extracellular acidification rate, Naïve = undifferentiated and no ethanol treatment, EtOH = undifferentiated and ethanol-treated, DIFF = differentiated and no ethanol treatment, and DIFF + EtOH = differentiated and ethanol-treated. Data represents average values using CD4+ T cells from 6 donors expressed as mean ± SEM. Significant differences (p<0.05) were determined by repeated measures 2-way ANOVA. *p ≤ 0.05.