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. 2022 Apr 7;17(5):1138–1153. doi: 10.1016/j.stemcr.2022.03.007

Figure 1.

Figure 1

OLFM4 gene expression increases concurrently with age

(A) UMAP visualizations of ages and clusters from EPCAM+ cells by scRNA-seq analysis from fetal tissue 47–132 days post-conception, corresponding to Figure S1.

(B) Feature plot and dot plot of OLFM4+ cells from extracted epithelium across time points corresponding to (A).

(C) Feature plot and dot plot of LGR5+ cells from extracted epithelium across time points corresponding to (A).

(D) H&E staining of fetal duodenum at 59, 91, 103, and 132 days post-conception and 65-year-old adult duodenum biopsy.

(E) Immunofluorescence (IF) protein staining for OLFM4 (pink) and ECAD (blue) with DAPI (gray) on fetal duodenum.

(F) Fluorescence in situ hybridization (FISH) staining for OLFM4 (green), LGR5 (red), and IF staining for ECAD (blue) with DAPI (gray) on fetal duodenum (n = 10 [n = 2 biological replicates per time point shown]).

Scale bars represent 100 μm.