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. 2022 Apr 7;17(5):1138–1153. doi: 10.1016/j.stemcr.2022.03.007

Figure 6.

Figure 6

Chronic γ-secretase inhibition promotes higher stem-cell survival in fetal enteroids younger than 20 weeks post-conception

(A) Experimental schematic for data presented in (B)–(D).

(B) FISH staining for OLFM4 (green) and LGR5 (red) with DAPI (gray) on fetal duodenum aged 58, 59, 70, 78, 91, 100, 110, 132, and 140 days post-conception, as well as 42- and 65-year-old adult duodenum (n = 11 [n = 1 biological replicates per time point]). Scale bar represents 50 μm.

(C) Stereomicroscope images of different passages for respective enteroids time points after LWRN and LWRN + DBZ treatments (n = 3 experimental replicates per time point from n = 1 biological replicate per time point). Scale bars represent 2 mm.

(D) Manual counting of enteroid number from respective time points and passages corresponding to (C) (n = 3 experimental replicates per time point from n = 1 biological replicate per time point). All statistics were analyzed with unpaired t tests by GraphPad Prism 7.0, and data were presented as the mean ± SEM. In all figures, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, and ∗∗∗∗p < 0.0001.