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. 2022 Apr 7;17(5):1138–1153. doi: 10.1016/j.stemcr.2022.03.007

Figure 7.

Figure 7

Primary fetal explant cultures respond to γ-secretase inhibition differently across fetal development

(A) Experimental schematic for data presented in (B)–(E).

(B) FISH staining for OLFM4 (red) and IF staining for ECAD (blue) with DAPI (gray) on fetal duodenum 59 days post-conception after LWRN (a) and LWRN + DBZ (b) treatments. Scale bars represent 200 μm.

(C) IF staining for KI67 (red) and ECAD (blue) with DAPI (gray) on fetal duodenum at 59 days post-conception after LWRN (a) and LWRN + DBZ (b) treatments. Scale bars represent 200 μm. (aʹ) represents high magnification of (a), and (bʹ) represents high magnification of (b) (n = 2 biological replicates). Scale bars represent 100 μm.

(D) FISH staining for OLFM4 (red) and IF staining for ECAD (blue) with DAPI (gray) on fetal duodenum at 125 days post-conception after LWRN (a) and LWRN + DBZ (b) treatments. Scale bars represent 200 μm.

(E) IF staining for KI67 (red) and ECAD (blue) with DAPI (gray) on fetal duodenum at 125 days post-conception after LWRN (a) and LWRN + DBZ (b) treatments. Scale bars represent 200 μm. (aʹ) represents high magnification of (a), and (bʹ) represents high magnification of (b) (n = 1 biological replicate). Scale bars represent 100 μm.