Figure 2.

ARdY fat distribution is determined by reduced progenitor seeding in nascent visceral fat

(A) Total stromal vascular cells (SVCs) in visceral fat of 18- to 21-day-old mice (n = 5–6).

(B) Total SVCs in subcutaneous fat of 18- to 21-day-old mice (n = 5–6).

(C) Total adipocyte precursor cells in visceral fat of 18- to 21-day-old mice (n = 4–6).

(D) Total adipocyte precursor cells in subcutaneous fat of 18- to 21-day-old mice (n = 4–6).

(E) SVCs per mg visceral fat (n = 5–6).

(F) SVCs per mg subcutaneous fat (n = 4–6).

(G) Adipocyte precursors per mg visceral fat (n = 5–6).

(H) Adipocyte precursors per mg subcutaneous fat (n = 4–6).

(I) Schematic of adipogenesis in vivo.

(J) Image showing how BrdU+ adipocytes are identified. Arrowhead shows DAPI+ adipocyte nucleus. Arrow shows BrdU+/DAPI+ adipocyte nucleus. Non-adipocyte nuclei are surrounded by stained plasma membranes, as described in Jeffery et al. (2015). Scalebar, 25 μm.

(K) New adipocyte formation from E18.5 to P18 (n = 3).

(L) New adipocyte formation from P4 to P18 (n = 3).

(M) Image of P4 testis and surrounding structures in ARdY and male mice. Arrow shows epididymal appendage. Statistical significance was determined by ordinary one-way ANOVA with Tukey’s multiple comparison’s test. AP, adipocyte precursor; F, female; A, ARdY; M, male; WT, wild type.