Figure 3.

Notch signaling is largely unaffected upon aux depletion

(A) Notch activation (by GBE + Su(H)-lacZ, white arrowheads) in control intestines (3 days). ISCs are GBE + Su(H)-lacZ^- cells (red arrowheads, the same as follows). GBE + Su(H)-lacZ channel is shown separately in black and white.

(B–D) GBE + Su(H)-lacZ⁺ cells in esg^ts>aux^RNAi intestines (white arrowheads, 3 days).

(E) Notch activation in control intestines (white arrowheads, 7 days).

(F–H) GBE + Su(H)-lacZ⁺ cells (white arrowheads) in esg^ts>aux^RNAi intestines.

(I) Quantification of the number and percentage of GBE + Su(H)-lacZ⁺ cells in control and esg^ts>aux^RNAi intestines, respectively. Means ± SDs are shown. n ≥ 15. ^∗∗∗∗p < 0.0001.

(J) Pdm1 in control intestines. Pdm1 channel is shown separately in black and white.

(K) Pdm1 in esg^ts>aux^v22 intestines (white arrowheads).

(L) Pdm1 in control MARCM clones (6 days ACI) (white arrowheads).

(M) Pdm1 in aux^v22 ISC MARCM clones (6 days ACI) (white arrowheads).

Scale bars, 20 μm (K–N, 10 μm).