Figure 6.

CLINT1 mediates a late stage of DENV infection. A, DENV entry measured via luciferase assay 6 h postinoculation of Huh7 cells transfected with the indicated siRNAs (Fig. 5A) with luciferase reporter DENV2 (MOI=5). B, DENV RNA replication measured in Huh7 cells by luciferase assays 24 and 72 h following cotransfection with both a Tet-inducible DNA-launched DENV replicon and a TET-ON plasmid and 24-h induction by doxycycline (GND is a replication-defective DENV). Data are normalized to signal at 24 h following Tet induction. C, DENV infectivity measured by luciferase assays following inoculation of naïve cells with lysates (intracellular) and supernatants (extracellular) harvested from Huh7 cells transfected with in vitro transcribed DENV2 RNA 48 h post-transfection. Data in (C) are normalized to siNT controls and shown as log₁₀. (A–C) Shown are means $\pm$ SD of results of representative experiments out of two conducted each with four replicates. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 relative to corresponding controls by one-way (A and C) or two-way (B) ANOVA with Dunnett’s post hoc tests. CLINT1, clathrin-interacting protein 1; DENV, dengue virus; MOI, multiplicity of infection; ns, nonsignificant.