FIGURE 7.

Fecal metabolite biomarkers for the diagnosis of TD broiler chickens. (A) Pearson correlation analysis between the differential microbiota and metabolites, differential metabolites and tibia-related parameters in feces of broiler chickens with TD. The asterisks indicate statistically significant differences and correspond to p < 0.05 (*), p < 0.01 (**), and p < 0.001 (***). A p-value higher than 0.05 (>0.05) represents statistically non-significant (N = 4). (B) Curves of ROC of differential metabolite biomarkers for diagnosis of TD in an independent test set. An AUC close to 1.0 indicates high sensitivity and specificity and statistical analysis was performed on the relative abundances of the three differential metabolites between groups by the Student’s t-test, a p-value < 0.05 was considered to be significant (N = 4). (C) ROC curve of serum ALP and TRACP for diagnosis of TD (N = 3).