TABLE 3.
Summary of biofuels production in R. palustris.
| Product | Characteristics of main strategies | Yield/titer | Culture conditions | References |
|---|---|---|---|---|
| Hydrogen | Screening of mutants that produce hydrogen constitutively, even in the presence of ammonium. | 332 μmol/mg protein | In PM medium with 4.5 mM p-coumarate by R. palustris mutant CGA574 | Rey et al. (2007) |
| Hydrogen | Disruption of the PHB synthesis gene phbC | 457 ml/L/day | In a 2.5 L bioreactor using organic acid synthetic wastewater | Yang and Lee (2011) |
| Hydrogen | Influence of light energy and electron availability on CH4 production, including providing cells with different substrates, using nongrowing cells, blocking electrons from entering the Calvin cycle, or blocking H2 uptake | 500 μmol/mg protein | Nongrowing cell suspensions were incubated in light for 10 days in PM with 20 mM acetate and 10 mM NaHCO3; R. palustris strain nifA* nifD V75AH201Q | Zheng and Harwood (2019) |
| Hydrogen | Evaluation of lighting systems, carbon sources | 4.92 mol H2/mol substrate | In 30 mM medium with 2 g/L butyrate under incandescent light | Hu et al. (2018) |
| Hydrogen | Effect of volatile fatty acids mixtures | 391 ml/g/day | 1.2 g/L acetic acid, 0.2 g/L propionic acid, and 0.05 g/L butyric acid | Cardena et al. (2017) |
| Hydrogen | — | 34 ml/g/h | 10 mM glycerol and 5 mM glutamate | Pott et al. (2013) |
| Hydrogen | Effects of light intensity, the concentrations of crude glycerol and glutamate | 6.69 mol/mol glycerol | A light intensity of 175 W/m2, 30 mM glycerol, and 4.5 mM glutamate, | Ghosh et al. (2012) |
| Hydrogen | Effect of different liming nitrogen regimes | 6 mol/mol glycerol | In RCV medium with 10 mM crude glycerol and 2 mM glutamate | Sabourin-Provost and Hallenbeck, (2009) |
| Hydrogen | Assessment of hydrogen production from agroindustrial residues and energy crops | 648.6 mg/L | In 100 ml photobioreactors with 100 ml wheat bran effluent under anaerobic conditions | Corneli et al. (2016) |
| Hydrogen | Application of immobilized-cell technology | 11.2 mmol/m2/h 70 ml/h flow rate 0.25 mol/mol glucose | Biofilm formed under 590 nm and 5,000 lx illumination | Liao et al. (2010) |
| Hydrogen | Application of a cell immobilization technique to a biofilm-based photobioreactor | 38.9 ml/L/h 0.2 mol/mol glucose | Illumination condition of 5,000 lux and 590 nm wavelength; glucose concentration is 0.12 M, the optimal pH = 7 and optimal temperature of influent liquid 25°C. | Tian et al. (2010) |
| Hydrogen | Latex coating immobilization on chromatography paper | 0.47 ± 0.04 mmol/m2/h | Incubated in the head-space of the Balch tubes | Gosse et al. (2012) |
| Hydrogen | Effect of osmoprotectants, temperature, humidity and O2 on H2 production in R. palustris coatings | 69.1% headspace | R. palustris coatings containing glycerol and sucrose after storage for 8–12 weeks at <5% relative humidity; production under argon 10 days post rehydration in PM (NF) medium | Piskorska et al. (2013) |
| Hydrogen | Blocking of the calvin cycle flux; effect of different substrate | 80 mol/mol C consumed | In PM with 10 mM butyrate in front of a 60-W light bulb at 30°C; strain R. palustris CGA679 | McKinlay and Harwood (2011) |
| Methane | nifD V75A and H201Q encoding a Mo-dependent nitrogenase; calvin cycle through genetic mutationΔcbbMLS; using nongrowing cells; effect of different substrates | 800 nmol/mg total protein | Nongrowing cell suspensions were incubated in light for 10 days in PM with 20 mM acetate and 10 mM NaHCO3; R. palustris strain nifA* nifD V75A H201Q | Fixen et al. (2016) |
| Methane | Influence of light energy and electron availability on CH4 production | 900 nmol/mg total protein | Nongrowing cell suspensions were incubated in light for 10 days in PM with 10 mM succinate and 10 mM NaHCO3; R. palustris strain nifA* nifD V75A H201Q | Zheng and Harwood (2019) |
| Methane | V-dependent nitrogenase (VnfD V57A H180Q ); Fe-only nitrogenase | 150 nmol/mg total protein (V); 400 nmol/mg total protein (Fe) | Non-growing cell suspensions incubated in light for 10 days in 10 ml NFM medium supplemented with 20 mM acetate and 10 mM NaHCO3 | Zheng et al. (2018) |
| Butanol | Overexpression of adhE2 gene | 1.5 mM; 0.03 g/L/day | Cultured in anaerobic butyrate Rhodospirillaceae medium | Doud et al. (2017) |