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. 2022 May 12;16:884296. doi: 10.3389/fncel.2022.884296

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Copyright © 2022 Wang, Wei, Yuan, Mao, Wang, Xing, Gu, Lin, Wang, Zhang and Xing.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Peripheral nerve injury can reduce the expression of FTO protein and FTO mRNA in mice ACC. (A,B) qRT-PCR and Western blot results showed that the levels of FTO mRNA and protein in ACC of mice decreased significantly 7, 14, 21, and 28 days after CCI (n = 4, *P < 0.05, ***P < 0.001 vs. Sham). (C) The immunofluorescence results of FTO in ipsilateral (Ipsi) and contralateral (Con) ACC 28 d after CCI (n = 4, **P < 0.01). (D) The IF double staining of FTO (red) and NeuN (a neuronal marker, green), GFAP (an astrocyte marker, green), or ox42 (a microglia marker, green) in contralateral ACC 28 days after CCI. Scale bar, 100 μm. FTO, fat mass and obesity-related protein.