FIGURE 5.

The mechanism of FTO affecting the expression of MMP-9. (A) qRT-PCR results of the expression of MMP-9 mRNA in ACC of Sham and CCI mice. (B,C) Western blot results of the expression of MMP-9, proBDNF, and mBDNF (*P < 0.05, **P < 0.01, ***P < 0.001 vs. Sham). (D) The representative images of the original hybridization of MMP-9 mRNA and co-labeling of FTO immunofluorescence in ACC. (E) The cellular expression patterns of FTO protein and MMP-9 protein. (F) RIP results of FTO and MMP-9 mRNA in ACC after CCI in mice (**P < 0.01 vs. Sham). (G) RIP results of m⁶A and MMP-9 mRNA in ACC after CCI in mice (***P < 0.001 vs. Sham).