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. 2022 May 12;16:883537. doi: 10.3389/fnana.2022.883537

FIGURE 10.

FIGURE 10

Fluorescent Sim1 and Fogx1 in the chicken forebrain. (A–C) Horizontal sections of the chicken embryonic forebrain, at E8, hybridized for Sim1 (magenta color) and immunostained for Foxg1 (green color) (left to right goes from bottom to top). Panels (D,D′) are a detail of the area squared in (B), showing Sim1 single-labeled (empty arrow) and Sim1/Foxg1 double-labeled (filled arrow) cells in the medial extended amygdala. Panels (E,E′) are a detail of the area squared in (A), showing Sim1 single-labeled (empty arrow) and Sim1/Foxg1 double-labeled (filled arrow) cells in BSTM3 or ventral to it. Panel (F) is a detail of the capsular central amygdala from the section shown in (C). Sim1 single-labeled (empty arrow) and Sim1/Foxg1 double-labeled (filled arrows) cells are observed. See text for more details. For abbreviations, see list. Scale bars: (A) = 200 μm (applies to A–C); (D) = 40 μm (applies to D–E′); (F) = 20 μm (applies to F,F′).