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. 2022 May 12;16:883537. doi: 10.3389/fnana.2022.883537

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Copyright © 2022 Metwalli, Abellán, Freixes, Pross, Desfilis and Medina.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescent double labeling of Otp and Fogx1 in chickens. (A–C) Horizontal section of the chicken embryonic forebrain, at E8, hybridized for Otp (magenta color) and immunostained for Foxg1 (green color). Panel (A) is at the peduncular prosomeric level, while (B,C) are at terminal levels. Arrows in (B,C) point to a stripe of Otp cells in the PO. Panels (D–E′) are details of the areas squared in (C) (D,E: merged channels; D′,E′: green channel). Panels (D,D′) show double-labeled cells in the SuPO (in the terminal TOH domain), and some Otp single-labeled (arrow) and Otp/Foxg1 double-labeled cells that spread into the PO. Panels (E,E′) show a prominent group of Otp single-labeled subpial cells, which seem to correspond to the terminal part of the supraoptic (SOt) nucleus. See text for more details. For abbreviations, see list. Scale bars: (A) = 320 μm (applies to A–C); (D) = 40 μm (applies to D–E′).