Figure 2.

Cpmer is critically required for proper CM differentiation

(A) FACS analysis and statistics for the percentage of Flk1⁺Pdgfrα⁺ cells. Data shown are the mean ± SEM, n = 3 independent experiments.

(B and C) qRT-PCR analysis of CPC (B) and CM (C) marker genes. Data shown are the mean ± SEM, n = 3 independent experiments.

(D) cTnT immunostaining (green) at the CM stage (left) and statistics for relative fluorescence intensity (right). Scale bar, 20 μm. Data shown are the mean ± SEM, n = 6 fields from 3 independent experiments.

(E) Percentage of spontaneously contracting EBs determined on days 6, 9, and 12 of EB differentiation. Data shown are the mean ± SEM, n = 3 independent experiments.

(F) qRT-PCR analysis of CM marker genes on day 12 of EB differentiation (EB-day 12). Data shown are the mean ± SEM, n = 3 independent experiments.

(G) FACS analysis and statistics of the percentage of Flk1⁺Pdgfrα⁺ cells. Data shown are the mean ± SEM, n = 3 independent experiments.

(H and I) qRT-PCR analysis of CPC (H) and CM (I) marker genes. Data shown are the mean ± SEM, n = 3 independent experiments.

(J) cTnT immunostaining (green) at the CM stage (left) and statistics for relative fluorescence intensity (right). Scale bar, 20 μm. Data shown are the mean ± SEM, n = 6 fields from 3 independent experiments.

(K) Percentage of spontaneously contracting EBs of EB differentiation. Data shown are the mean ± SEM, n = 3 independent experiments.

(L) qRT-PCR analysis of CM marker genes at EB-day 12. Data shown are the mean ± SEM, n = 3 independent experiments. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001 (versus WT); Student’s t test.

See also Figure S2.