TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Pseudomonas aureofaciens strains | ||
| 30-84 | Phz+ Rifr wild type | W. W. Bockus |
| 30-84Z | Phz− RifrphzB::lacZ genomic fusion | 16 |
| 30-84R | Phz− RifrphzR::Tn5lacZ genomic fusion | 16 |
| 30-84Ice | Phz− RifrphzB::inaZ genomic fusion | 26 |
| 30-84I | Phz− RifrphzI::Kmr | 27 |
| 30-84Z/I | Phz− RifrphzB::lacZ and phzI::Kmr genomic fusions | 27 |
| 30-84W | Phz− Rifr spontaneous gacA mutant | This study |
| 30-84.A2 | Phz− Rifr Kmr spontaneous gacS mutant | This study |
| 30-84.gacA | Phz− RifrgacA::Kmr | This study |
| Escherichia coli strains | ||
| DH5α | F− recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA1 Δ(argF-lacZYA)I169 φ80lacZ ΔM15 | GIBCO-BRL |
| HB101 | F− hsdS20 (rB−mB−) supE44 recA1 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-5 | GIBCO-BRL |
| Plasmids | ||
| pDW7311 | pLAFR3 containing ExoIII-truncated phzI of strain 30-84 | This study |
| pDW7311uidA | pLAFR3 containing a phzI::uidA transcriptional reporter fusion | This study |
| pEMH97 | pLAFR3 containing gacS from P. syringae pv. syringae B728a | 24 |
| pHP45Ω-Kmr | pUC18 containing Kmr cassette | 4 |
| pJEL5771 | pLAFR3 containing the gacS homolog apdA of P. fluorescens Pf-5 | 1 |
| pLAFR3 | IncP1 Tetr cos+ rlx+ | 23 |
| pLSP20H-2.7#7 | pIC20H containing 2.7-kb PstI fragment of pLSP259 with phzI/phzR | 16 |
| pLSP259 | pLAFR3 containing phzI, phzR, and the phenazine biosynthetic locus on a 20.4-kb EcoRI fragment of strain 30-84 chromosomal DNA | 17 |
| pLSP259Tn5lac#42 | pLAFR3 containing Tn5lacZ insertion within phzR in pLSP259 | 16 |
| pLSP619 | pLAFR3 containing 33 kb of strain 30-84 chromosomal DNA as multiple EcoRI fragments | This study |
| pME3066 | pLAFR3 containing gacA from P. fluorescens CHA0 | 9 |
| pRK2013 | IncP1 tra oriE1 Kmr | 2 |
| pSTC110 | pLAFR3 containing 15-kb EcoRI fragment of pLSP619 | This study |
| pSTC121 | pLAFR3 containing 4.5-kb EcoRI-PstI gacA fragment of pSTC110 | This study |
| pSTC122 | pLAFR3 containing 2.2-kb KpnI-PstI gacA fragment of pSTC121 | This study |
| pSTC140 | pUC18 containing 2.2-kb KpnI-PstI gacA fragment of pSTC121 | This study |
| pSTC161 | pLAFR3 containing gacA disrupted by the Kmr cartridge of pHP45Ω-Kmr | This study |
| pUC18 | ColE1 Apr | 28 |
| pWM6 | ColE1 vector containing uidA transcriptional fusion cassette | 10 |