Figure 2.

CDCA5 knockdown inhibits the proliferation of MDA-MB-231 cells. (A) Western blotting and (B) reverse transcription-quantitative PCR were performed to detect CDCA5 expression. (C) Cell Counting Kit-8 assay was used to detect the level of cell proliferation. (D) Colony formation assay was used to detect the colony formation ability of the cells. (E) The expression levels of the proliferation-related proteins Ki67 and PCNA were reduced upon CDCA5 silencing, as indicated by western blotting. *P<0.05, **P<0.01 and ***P<0.001 vs. control; ^#P<0.05, ^##P<0.01 and ^###P<0.001 vs. shRNA-NC. CDCA5, cell division cycle-associated 5; sh, short hairpin; NC, negative control; PCNA, proliferating cell nuclear antigen; OD, optical density.