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. 2022 May 3;25(6):209. doi: 10.3892/mmr.2022.12725

Figure 4.

Figure 4.

CDCA5 knockdown downregulates PDS5A expression in breast cancer cells. (A) starBase website (https://starbase.sysu.edu.cn/) indicated that PDS5A was upregulated in tissues of patients with breast cancer compared with normal tissues adjacent to the cancer. (B) Gene Expression Profiling Interactive Analysis database (http://gepia.cancer-pku.cn/) indicated that the expression of CDCA5 and PDS5A in patients with breast cancer was positively correlated. (C) Western blotting and (D) reverse transcription-quantitative PCR were used to detect the expression level of PDS5A in MDA-MB-231 cells. (E) Co-IP assay further indicated that CDCA5 could bind to PDS5A. (F) Western blotting indicated that knocking down the expression of CDCA5 inhibited the expression of PDS5A. *P<0.05, **P<0.01 and ***P<0.001 vs. control or MCF-10A; ##P<0.01 vs. shRNA-NC. CDCA5, cell division cycle-associated 5; sh, short hairpin; NC, negative control; PDS5A, PDS5 cohesin-associated factor A; IP, immunoprecipitation; log, logarithm; BRCA, breast cancer. TPM, Transcripts Per Million.