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. 2022 May 12;13:861655. doi: 10.3389/fimmu.2022.861655

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Copyright © 2022 Ma, Zhan, Bartolomé-Cabrero, Ying, Asano, Huang, Xiao and González-Martín

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of the top hit shRNAs in the in vitro functional screen. (A) Representative flow cytometry histograms showing apoptosis of WEHI-231 transduced with retroviruses encoding shRNAs or empty vector (EV), stimulated for 72 hours with 2µg/ml anti-IgM (Stim) or left unstimulated (US), followed by flow cytometry analysis of active caspase 3 (Casp3). (B) Frequency of active caspase-3-positive (Casp3⁺) cells among total cells in (A). Every independent experiment included an internal WEHI-EV control. A representative WEHI-EV control experiment is shown. Results are pooled from 3 independent experiments for EV, Phip-F1, Nsd1-E11, Ciita-E6, Dcp2-A9, Cdon-B5 and Cdc14a-A5, 4 for Gpatch8-B3, Uhmk1-F10, Ankrd52-C2, Ube2w-A10 and Snx27-E6, and 5 for B4galt5-A4 and Mmp15-H7. Graph bars indicate mean + SD.