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. 2022 May 12;13:894534. doi: 10.3389/fimmu.2022.894534

Figure 2.

Figure 2

Dynamics of pathogen loads during SARS-CoV-2 infection and pneumococcal coinfection. Lung bacterial loads (CFU/lung) (A), blood bacterial loads (B), and lung viral loads (PFU/lung) (C) in female (circles) and male (triangles) mice infected with SARS-CoV-2 (250 PFU; white) followed 103 CFU D39 at 3 d (yellow), 5 d (magenta), or 7 d (cyan) pvi. Each symbol represents a single mouse and the mean ± standard deviation (SD) are for combined male and female groups. Significant differences are indicated by ns, not significant; *P < 0.05; ****P < 0.0001. For bacterial titers, comparison was with the inoculum (dotted line). (D, E) Representative immunohistochemical (IHC) staining for SARS-CoV-2 nucleocapsid protein in whole lung sections following (24 h pbi) infection with SARS-CoV-2 (250 PFU) then PBS or 103 CFU D39 at 3 d (D) or 5 d (E) pvi. (F) Representative lung sections stained with H&E, SARS-CoV-2 nucleocapsid protein, or pneumococcus from infection with SARS-CoV-2 (250 PFU) followed by 103 CFU D39 at 5 d pvi. Lesions with perivascular inflammatory cell infiltration are indicated by arrows; blood vessel (BV). Scale bar = 100 µm.