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. 2022 Feb 28;34(6):2286–2308. doi: 10.1093/plcell/koac073

Figure 1.

Figure 1

cop1 mutants are less sensitive to ABA-inhibited seedling growth in darkness. A, Germination rate measurements. Hydrated seeds for Col-0 and the cop1 mutants were sown on MS medium containing various concentrations of ABA, and then grown in darkness. Seed germination was defined as the first sign of radicle tip emergence and scored daily until day 6. Error bars represent the standard deviation (sd) of three independent sets of seeds, each set containing 50 seeds. *P < 0.05, **P < 0.01, and ***P < 0.001 (Student’s t test; Supplemental Data Set S1) for the indicated pairs of seeds. B, Growth of cop1 seedlings is insensitive to ABA. Col-0 and cop1 seedlings were grown vertically on MS medium with the indicated ABA concentrations in darkness (D) for 5 days. Bar = 1 cm. C, Seedling establishment of cop1 mutants is less sensitive to ABA. Col-0 and cop1 seedlings were grown horizontally on MS medium or MS medium containing 0.5-µM ABA in darkness for 5 days. Bar = 1 cm. D, Seedling establishment rate measurements. Col-0 and cop1 seedlings were grown horizontally on MS medium or MS medium containing 0.5-µM ABA in darkness. Error bars represent sd of three independent pools of seedlings, each pool containing 50 seedlings. *P < 0.05, **P < 0.01, and ***P < 0.001 (Student’s t test; Supplemental Data Set S1) for the indicated pairs of seedlings.