Roles of BLI in seed maturation. A, Mature seed phenotypes of WT, bli mutants and bli transgenic complementation plants. bli-1 and bli-11 are two independent BLI loss-of-function mutant lines. pBLI:BLI/bli-1#1 and pBLI:BLI/bli-1#2 indicate two independent bli-1 complementation plant lines. Bar = 1 mm. B, Seed sizes of lines with different BLI expression levels. Values are means ± SD (N = 3); each of the three assays for each biological replicate contained 50 seeds. Seed size does not significantly differ when labeled with the same letter, as determined by Tukey’s HSD test (P < 0.05). C, 100-grain weight of plants with different BLI genomic constructs. Data shown are means ± standard deviation (sd) (N = 3). Seed mass does not significantly differ when labeled with the same letter, as determined by Tukey’s HSD test (P < 0.05). D, Mature embryo phenotypes of different BLI complementation plants. Bars = 100 μm. E, Observation of the maturation process of bli seeds. Bars = 1 mm.