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. 2022 Mar 16;34(6):2222–2241. doi: 10.1093/plcell/koac093

Figure 6.

Figure 6

Malate content analysis and rescue of the ead1 phenotype in maize. A, Malate contents in the apical, middle and basal parts of developing ears in WT and ead1. About 10-mm ears were divided into two parts (apical and basal parts); the remaining ears were divided into three parts (apical, middle, and basal parts). Pairwise comparisons were conducted between WT and ead1 in each part. Ap, apical; Md, middle; Bs, basal. Values are means, error bars indicate the sd of three biological replicates; **P < 0.01 as determined by two-tailed Student’s t test between WT and ead1. B and C Rescue of the ead1 phenotype by exogenous malate supply. Representative ear phenotype (B) and corresponding average EL (C) of ead1 after exogenous malate supplementation by spraying (indicated by S), irrigation of roots (indicated by Ir) or injection of the stem-adhered ear (indicated by In) with 5-mM malate. Scale bar = 5 cm. Values are means, error bars indicate sd (n = 15). Different letters indicate significant difference at P < 0.05 determined by Tukey’s HSD test.