Figure 8.

Contribution of EAD1 to EL. A–D, EAD1-overexpression line (OE1) produces longer ears than a nontransgenic line (B104). A, Typical ears at the full-ripe stage. B, Relative EAD1 transcript levels, as analyzed by RT-qPCR. Values are means ± sd (n = 3). C and D, Average EL (C) (n = 60) and KNR (D) (n = 25) in OE1 line and B104. **P < 0.01 as determined by two-tailed Student’s t test. E–J, Effect of different EAD1 alleles on EL. E, Representative ears in two inbred lines with the A₂A₂ (LS3) or A₁A₁ (HN321) alleles of EAD1. F, Distribution of EL in the F₂ segregating population derived from the HN321 × LS3 cross. G, Average EL of the A₁A₁ and A₁A₂ genotypes is significantly higher than that of the A₂A₂ genotype. H, Ears from two inbred lines with the A₃A₃ (LS84) or A₁A₁ (HN321) alleles of EAD1. I, Distribution of EL in the F₂ segregating population derived from the HN321 × LS84 cross. J, Average EL of the A₁A₁ and A₁A₃ genotypes is significantly higher than that of the A₃A₃ genotype. Boxplots represent the range between first and third quartiles (25%–75%); whiskers, full data range; horizontal center line, median. Different letters in (G and J) indicate significant difference at P < 0.05 determined by Tukey’s HSD test. Scale bars = 5 cm in (A, E, and H).