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. 2022 Mar 2;34(6):2188–2204. doi: 10.1093/plcell/koac078

Figure 5.

Figure 5

Daytime temperature affects nuclear levels of PIF4 and HY5 during the night. A–D, Nighttime kinetics of nuclear fluorescence intensity driven in hypocotyl cells by pPIF4:PIF4-GFP (A and B) or pHY5:HY5-YFP (C and D). See Supplemental Figure S2 for nuclear fluorescence driven by pPIF4:PIF4-GFP in cotyledons. E, Nighttime kinetics of luminescence driven by pPIF4:PIF4-LUC in whole seedlings. F, Luminescence driven by pPIF4:PIF4-LUC in isolated hypocotyls harvested at ZT = 14 h. Confocal images or luminescence values were recorded during the night (starting at ZT = 10 h), as affected by four combinations of daytime temperature and nighttime temperature. All seedlings received shade during the day and EOD FR. (A, C, and E) Data are means ± se of three replicate boxes with seedlings. F, Box plots show median, interquartile range 1–3 and the maximum–minimum interval of 10–14 replicate boxes with seedlings. B and D, Representative confocal images at ZT = 14 h. Scale bar = 35 µm. See Supplemental File S1, G–I for detailed statistics of (A, C, and E). In (F), the asterisks indicates significant differences in t test (**P < 0.01).