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. 2022 Mar 2;34(6):2188–2204. doi: 10.1093/plcell/koac078

Figure 7.

Figure 7

Effects of temperature on end-of-day PIF4 promoter activity and PIF4 protein abundance require ELF3. A and B, Time course of daytime LUC activity driven by pPIF4:PIF4-LUC (A) or pPIF4:LUC (B) in the Col-0 and elf3-8 background, as affected by 10°C or 28°C. C, Ratio between the LUC signals driven by pPIF4:PIF4-LUC and pPIF4:LUC. See Supplemental Figure S3 for protein blots supporting posttranscriptional effects of temperature on PIF4 abundance. D, Luminescence driven by pPIF4:LUC in isolated hypocotyls harvested from Col-0 and elf3-8 seedlings at ZT = 14 h. All seedlings received shade during the day and EOD FR. A and B, Data are means ± se of three plates with seedlings. D, Box plots show median, interquartile range 1–3 and the maximum–minimum interval of 24–30 replicate plates seedlings; asterisk indicates significant differences in t tests (*P < 0.05). See Supplemental File S1, L and M for detailed statistics of (A and B).